HIGH-LEVEL FLUORESCENCE LABELING OF GRAM-POSITIVE PATHOGENS.

High-level fluorescence labeling of gram-positive pathogens.

Fluorescence labeling of bacterial pathogens has a broad range of interesting applications including the observation of living bacteria within host cells.We constructed a novel vector based on the E.coli streptococcal shuttle plasmid pAT28 that can propagate in numerous bacterial Wooden species from different genera.The plasmid harbors a promoterle

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The dCas9-based genome editing in Plasmodium yoelii

ABSTRACTGenetic editing is a powerful tool for functional characterization of genes in various organisms.With its simplicity and specificity, the CRISPR-Cas9 technology has become a popular editing tool, which introduces site-specific DNA double-strand breaks (DSBs), and then leverages the endogenous repair pathway for DSB Rifle Bolt Pouch repair v

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